Mike Tyson Punches Jake Paul At Vicious Fight Night Stare Down: Video
The heated press conference before their exhibition boxing match in February saw tempers flare as the former heavyweight champion took a swing at the YouTuber.
The pair were face-to-face for the final time before their eagerly-anticipated bout when tempers flared.
In footage shared on social media, Tyson can be seen making physical contact with Paul as security intervened to separate the two.
Paul later tweeted: "My respect for Tyson diminished."
Tyson, 55, has not fought professionally since facing Roy Jones Jr in an exhibition bout in 2020.
Paul, 26, has a professional boxing record of 6-0 with four knockouts.
The pair are due to meet in the ring in Glendale, Arizona, on February 25.
The fight is being billed as a grudge match, with Paul having repeatedly called out Tyson in recent months.
Tyson has accused Paul of being disrespectful and claims he is not taking the fight seriously.
The bad blood between the pair boiled over at the press conference, with Tyson appearing to lose his cool.
In the footage, Tyson can be seen leaning into Paul's face and making physical contact with him.
Security guards quickly intervened to separate the two, but the incident has cast doubt over whether the fight will go ahead as planned.
Paul later tweeted: "My respect for Tyson diminished."
It remains to be seen whether the incident will have any impact on the fight, but it has certainly added to the hype surrounding the event.